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Describe Transcription in Prokaryotes?...

Describe Transcription in Prokaryotes?

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The synthesis of RNA from DNA is known as transcription.
Transcription in Prokaryotes
DNA dependent RNA polymerase is the single enzyme that catalyses the transcription of all types of bacterial RNA. This process occurs in following three steps
(i) Initiation RNA polymerase binds to promoter and initiates transcription by associating transiently with initiation sigma factor `(sigma)`. It uses nucleoside triphosphates as substrate and polymerises in a template depended fashion obeying the rule of complementarity. It also facilitates opening of the helix and continues elongation.
(ii) Elongation Chain elongation proceeds in the `6+3` direction and the transcription bubble travels with the RNA polymerase. The RNA polymerase after initiation of RNA loses the factor, but continues the polymerisation of ribonucleotides to form RNA.
(iii) Termination When the RNA polymerase reaches the terminator region of DNA, the nascent RNA separate and the RNA polymerase fall off resulting in termination of transcription. RNA polymerase associates transiently with the termination rho factor `(rho)` to terminate the transcription.
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ARIHANT PRAKASHAN-VERY SIMILAR TEST 2-Section - B (Zoology)
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