What is Southern blotting?

What is the first step in Southern Blotting technique ?

The figure shows the restriction enzyme cutting sites (R1-R3) in wild type (n) and mutant (n) gene. If a radioactively labelled probe (that hybridises at a sequence close to R1) is used for detecting the presence of DNA fragments after gel electrophoresis and Southern blotting, which of the following band patterns will yout expect ? Note : L1 : wild type DNA, L2: mutant DNA

Write brief notes on the Southern blotting .

Differentiate between the following Southern blotting and Western blotting.

Process used for amplification or multiplication of DNA in DNA fingerprinting is 1) polymerase chain reaction 2) southern blotting 3) northern blotting 4) None of these

Southern blotting is done for :

Southern blotting can't be performed without :

Study following statements regarding the preparation of herbarium sheets. (i) Plant should be collected in flowering stage. (ii) Every detail regarding the plant such sa locality, ecological conditions, vegetative and floral characters, etc. should be noted. (iii) Plants are evenly pressed by unfolding all the plant parts between blotting papers (or newspapers) with the help of plant pressers. (iv) Blotting papers need not be changed until the plant gets dried. (v) After drying, the plant specimen is carefully mounted/pasted on the herbarium sheets. (vi) The herbarium sheet is labelled on the lower right hand corner representing the number of plant specimen, data of collection, etc. Which of the above statements is/are correct ?