To form embryo in vitro, the male gamete is transferred into female gamete directly. Such technique is called

Options

A. IUI
B. IUT
C. ICSI
D. GIFT

Answer

To answer the question, "To form an embryo in vitro, the male gamete is transferred into the female gamete directly. Such technique is called," we need to identify the correct reproductive technology from the options provided.

### Step-by-Step Solution:

1. **Understanding In Vitro Fertilization (IVF)**:
- The term "in vitro" refers to processes that occur outside the living organism, typically in a laboratory setting. In this context, it means that the embryo is formed outside the female body.

2. **Identifying Male and Female Gametes**:
- The male gamete is the sperm, and the female gamete is the ovum (egg). The process involves the direct transfer of the sperm into the ovum.

3. **Recognizing the Technique**:
- The technique where the sperm is directly injected into the cytoplasm of the ovum is known as **Intracytoplasmic Sperm Injection (ICSI)**. This method is specifically designed for cases where traditional fertilization methods may not be successful.

4. **Reviewing Other Options**:
- **IUI (Intrauterine Insemination)**: This technique involves placing sperm directly into the uterus but does not involve direct injection into the egg.
- **IUT (Intrauterine Transfer)**: This refers to transferring a fertilized egg (zygote) into the uterus after fertilization has occurred, not the direct injection of sperm.
- **GIFT (Gamete Intra-Fallopian Transfer)**: This involves transferring gametes into the fallopian tubes, not direct injection into the egg.

5. **Conclusion**:
- Based on the definitions and descriptions of the techniques, the correct answer to the question is **ICSI (Intracytoplasmic Sperm Injection)**.

### Final Answer:
The technique is called **ICSI (Intracytoplasmic Sperm Injection)**.