Disease free plants are obtained from

Options

A. Anther culture
B. Ovary culture
C. Shoot-apex culture
D. Root-apex culture

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks where disease-free plants are obtained from. This implies that we need to identify a method or technique that is specifically used to produce plants that are free from diseases.

2. **Analyzing the Options**: The options provided are:
- Anther culture
- Ovary culture
- Shoot apex culture
- Root apex culture

3. **Defining Each Option**:
- **Anther Culture**: This involves culturing the anthers of flowers to produce haploid plants, but it is not specifically aimed at producing disease-free plants.
- **Ovary Culture**: This involves culturing the ovary of a flower, which is also not primarily focused on disease-free plant production.
- **Shoot Apex Culture**: This method involves culturing the shoot tip of a plant, which contains meristematic tissue. This tissue is responsible for the growth of new cells and is typically free from diseases.
- **Root Apex Culture**: This involves culturing the root tips, but similar to the other options, it does not specifically focus on producing disease-free plants.

4. **Identifying the Correct Answer**: Among the options, **Shoot Apex Culture** is the most appropriate choice because the shoot apex contains meristematic tissue that is less likely to carry diseases. Since it is a region of active growth, it can be used to regenerate a new plant that is disease-free.

5. **Conclusion**: Therefore, the correct answer to the question is **Shoot Apex Culture**.

**Final Answer**: Disease-free plants are obtained from **Shoot Apex Culture**.

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