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What are the properties of a good vector...

What are the properties of a good vector?
A. It should be ideally more than 10 kb in size B. It is should be able to replicate autonomously
C. it should have suitable marker genes D. It should not be easy to isolate and purify.

A

A,B

B

B,C

C

C,D

D

A,B,C,D

Text Solution

AI Generated Solution

The correct Answer is:
To determine the properties of a good vector, we can analyze each option provided in the question. ### Step-by-Step Solution: 1. **Evaluate Option A: Size of the Vector** - The statement suggests that a good vector should be ideally more than 10 kb in size. However, this is not a strict rule. Vectors can be effective even if they are smaller than 10 kb, as long as they can efficiently take up and deliver the DNA. Therefore, this option is **not a necessary property** of a good vector. 2. **Evaluate Option B: Autonomous Replication** - A good vector should be able to replicate autonomously. This means that the vector must contain all the necessary elements (such as origin of replication) to replicate independently within the host cell without relying on the host's nuclear genes. This is indeed a **crucial property** of a good vector. 3. **Evaluate Option C: Suitable Marker Genes** - The presence of suitable marker genes is essential for the selection of recombinant cells. Marker genes help in identifying which cells have successfully taken up the vector with the gene of interest. This property is also a **key characteristic** of a good vector. 4. **Evaluate Option D: Isolation and Purification** - The statement suggests that a good vector should not be easy to isolate and purify. This is misleading; in fact, a good vector should be easy to isolate and purify to facilitate further experiments and applications. Thus, this option is **not a property** of a good vector. ### Conclusion: From the evaluation: - **Correct Properties**: B (should replicate autonomously) and C (should have suitable marker genes). - **Incorrect Properties**: A (size is not a strict requirement) and D (should be easy to isolate and purify). ### Final Answer: The properties of a good vector are: - B. It should be able to replicate autonomously. - C. It should have suitable marker genes.
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