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A. In elution the separated bands of DNA...

A. In elution the separated bands of DNA are cut out from agarose gel and extracted from the gel piece.
B. E. coli cloning vector PBR322 shows several restriction, Ori, antibiotic resistance genes and Rop.
C. The down stream processing and quality control testing vary from produced to product.
D. Competent bacterial cell cannot take up the plasmid.

A

All are correct

B

All are incorrect

C

Except D all are correct

D

Only D is correct

Text Solution

AI Generated Solution

The correct Answer is:
To analyze the statements provided in the question, we will evaluate each one step by step. ### Step-by-Step Solution: **Step 1: Evaluate Statement A** - **Statement A**: "In elution the separated bands of DNA are cut out from agarose gel and extracted from the gel piece." - **Analysis**: This statement is correct. In gel electrophoresis, DNA fragments are separated based on size, and the bands can be cut out from the agarose gel for further analysis or use. The process of extracting DNA from the gel is known as elution. **Step 2: Evaluate Statement B** - **Statement B**: "E. coli cloning vector PBR322 shows several restriction, Ori, antibiotic resistance genes and Rop." - **Analysis**: This statement is also correct. PBR322 is a well-known plasmid vector used in molecular cloning. It contains multiple restriction sites, an origin of replication (Ori), and genes that confer antibiotic resistance, making it a versatile tool for cloning in E. coli. **Step 3: Evaluate Statement C** - **Statement C**: "The downstream processing and quality control testing vary from produced to product." - **Analysis**: This statement is correct as well. Downstream processing involves the purification and analysis of the product after fermentation or expression, and the quality control measures can differ significantly based on the type of product being produced (e.g., proteins, enzymes, etc.). **Step 4: Evaluate Statement D** - **Statement D**: "Competent bacterial cell cannot take up the plasmid." - **Analysis**: This statement is incorrect. Competent bacterial cells are specifically treated to be able to take up plasmid DNA from their environment. If a bacterial cell cannot take up plasmid DNA, it is referred to as a non-competent cell. ### Conclusion: - Statements A, B, and C are correct. - Statement D is incorrect. - Therefore, the correct combination is that all statements are correct except for D. ### Final Answer: The correct option is that A, B, and C are correct, while D is incorrect. ---
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Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

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Draw a schematic diagram of the E. coli cloning vector Pbr322 and mark the following in it : (a) ori (b) rop (c) ampicillin resistance gene (d) tetracycline resistance gene (e) restriction site BamHI (f) restriction site EcoRI

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