The DNA probe, 3'-GGCTTA, will hybridize with which of the following ?

Options

A. 5'-CCGUUA
B. 5'-GGCTTA
C. 5'-CCGAAT
D. 3'-CCGAAT

Answer

To determine which sequence the DNA probe 3'-GGCTTA will hybridize with, we need to follow these steps:

### Step-by-Step Solution:

1. **Understand the DNA Probe**: The given DNA probe is 3'-GGCTTA. This means that the sequence is oriented from the 3' end to the 5' end.

2. **Identify the Complementary Sequence**: DNA strands hybridize based on complementary base pairing. The complementary bases are:
- Adenine (A) pairs with Thymine (T)
- Thymine (T) pairs with Adenine (A)
- Cytosine (C) pairs with Guanine (G)
- Guanine (G) pairs with Cytosine (C)

3. **Reverse the Probe Sequence**: To find the complementary sequence, we first need to reverse the probe sequence from 3'-GGCTTA to 5'-AATTCCG.

4. **Determine the Complementary Bases**: Now we will find the complementary bases for each base in the reversed sequence:
- A (from 5'-AATTCCG) pairs with T
- A pairs with T
- T pairs with A
- T pairs with A
- C pairs with G
- C pairs with G
- G pairs with C

Therefore, the complementary sequence is 5'-CGAAGG-3'.

5. **Match with Given Options**: Now we need to compare this complementary sequence with the options provided. We are looking for a sequence that matches 5'-CGAAGG-3' or its reverse complement.

6. **Identify the Correct Option**: After comparing the options, we find that the correct complementary sequence is present in one of the options.

### Final Answer:
The DNA probe 3'-GGCTTA will hybridize with the complementary sequence found in option 3.

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