`{:(,"Column I",,"Column II"),(I.,"Escherichia coli",(A),"nif gene"),(II.,"Bacillus thuringiensis",(B),"Interferon"),(III.,"Rhizobium meliloti",(C),"Bt toxin"),(IV.,"Pseudomonas putida",(D),"Bioremediation"):}`

Options

A. I - B, II - A, III - D, IV - C
B. I - B, II - A, III - C, IV - D
C. I - B, II - C, III - A, IV - D
D. I - D, II - A, III - B, IV - C

Answer

To solve the matching question, we need to correctly pair the organisms listed in Column I with their respective functions or products in Column II. Here’s a step-by-step breakdown of the solution:

### Step 1: Identify the first organism - Escherichia coli
- **Escherichia coli (E. coli)** is a bacterium commonly used in biotechnology. It is known for its role in producing **Interferon** (a type of protein used in the treatment of various diseases).
- **Match:** E. coli → (B) Interferon

### Step 2: Identify the second organism - Bacillus thuringiensis
- **Bacillus thuringiensis (B. thuringiensis)** is known for producing a protein called **Bt toxin**, which is used as an insecticide in agriculture.
- **Match:** B. thuringiensis → (C) Bt toxin

### Step 3: Identify the third organism - Rhizobium meliloti
- **Rhizobium meliloti** is a bacterium that forms symbiotic relationships with legumes and is involved in nitrogen fixation. The **nif gene** is crucial for this process.
- **Match:** Rhizobium meliloti → (A) nif gene

### Step 4: Identify the fourth organism - Pseudomonas putida
- **Pseudomonas putida** is known for its ability to degrade environmental pollutants, making it useful in **bioremediation** processes.
- **Match:** Pseudomonas putida → (D) Bioremediation

### Final Matching Summary:
- I. Escherichia coli → (B) Interferon
- II. Bacillus thuringiensis → (C) Bt toxin
- III. Rhizobium meliloti → (A) nif gene
- IV. Pseudomonas putida → (D) Bioremediation

### Final Answer:
- I - B
- II - C
- III - A
- IV - D

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