Which of the following enzyme did not affect transformation in Griffith experiment :-

Options

A. Proteases
B. RNAases
C. DNAases
D. 1 & 2 both

Answer

To solve the question regarding which enzyme did not affect transformation in Griffith's experiment, we can follow these steps:

### Step 1: Understand Griffith's Experiment
Griffith's experiment was conducted using two strains of Streptococcus pneumoniae: the smooth strain (S) which was virulent and the rough strain (R) which was non-virulent. He discovered that when he injected mice with heat-killed S strain and live R strain, the mice developed pneumonia and died. This indicated that some "transforming principle" from the dead S strain transformed the R strain into a virulent form.

### Step 2: Identify the Role of DNA
The "transforming principle" was later identified as DNA. In Griffith's experiment, the transformation occurred because the DNA from the dead S strain was taken up by the live R strain, allowing it to express the virulence characteristics.

### Step 3: Analyze the Enzymes Listed
The question asks which enzyme did not affect transformation. The relevant enzymes are:
- **DNAse**: This enzyme breaks down DNA. If DNA is broken down, transformation cannot occur.
- **RNAse**: This enzyme breaks down RNA. Since RNA is not the transforming principle in this experiment, it does not affect transformation.
- **Protease**: This enzyme breaks down proteins. Similar to RNAse, protease does not affect the transformation since proteins are not the transforming principle.

### Step 4: Conclusion
Based on the analysis:
- **DNAse** would affect transformation because it degrades DNA.
- **RNAse** and **Protease** do not affect transformation because they do not act on DNA.

Thus, the enzymes that did not affect transformation in Griffith's experiment are **RNAse and Protease**.

### Final Answer
The enzymes that did not affect transformation in Griffith's experiment are **RNAse and Protease**.

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