In recombinant DNA technology, which of the following enzymes act as molecular glue?

Options

A. lysozyme
B. DNA polymerase
C. `T_4` ligase
D. Alkaline phosphatase

Answer

To solve the question regarding which enzyme acts as a molecular glue in recombinant DNA technology, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**: The question is about recombinant DNA technology and specifically asks for an enzyme that acts as a "molecular glue."

2. **Identify the Options**: The options provided are:
- Lysozyme
- DNA polymerase
- T4 ligase
- Alkaline Phosphatase

3. **Analyze Each Option**:
- **Lysozyme**: This enzyme is known for its antibacterial properties and is found in saliva and tears. It does not function as a molecular glue.
- **DNA Polymerase**: This enzyme synthesizes DNA molecules from nucleotides, but it does not join DNA strands together.
- **T4 Ligase**: This enzyme is specifically known for its ability to join two DNA strands together by forming phosphodiester bonds. It is often referred to as a molecular glue in recombinant DNA technology.
- **Alkaline Phosphatase**: This enzyme removes phosphate groups from molecules, but it does not have a role in joining DNA strands.

4. **Conclusion**: Based on the analysis, T4 ligase is the enzyme that acts as a molecular glue in recombinant DNA technology.

### Final Answer:
The enzyme that acts as a molecular glue in recombinant DNA technology is **T4 ligase**.

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