Which of the following enzyme is not used for extraction of desired gene from a bacterium?

Options

A. Lysozyme
B. Ribonuclease
C. Chitinase
D. Protease

Answer

To solve the question regarding which enzyme is not used for the extraction of a desired gene from a bacterium, we can follow these steps:

### Step 1: Understand the Question
The question asks us to identify an enzyme that is not involved in the extraction of a desired gene specifically from a bacterium.

### Step 2: Analyze the Options
We have four options to consider:
1. Lysosine
2. Ribonuclease
3. Chitinase
4. Protease

### Step 3: Evaluate Each Option
- **Lysosine**: This enzyme is known to break down bacterial cell walls, making it useful in the extraction of genetic material from bacteria.

- **Ribonuclease**: This enzyme degrades RNA and can be used in the process of isolating DNA from bacterial cells by removing RNA contaminants.

- **Chitinase**: This enzyme breaks down chitin, which is a component of fungal cell walls. It is not typically used for bacterial gene extraction.

- **Protease**: This enzyme breaks down proteins and is often used in the extraction process to remove proteins that may interfere with the isolation of DNA.

### Step 4: Identify the Correct Answer
From the analysis, we can see that Chitinase is specifically associated with the breakdown of chitin in fungal cell walls and is not used for extracting genes from bacteria. Therefore, the correct answer is **Chitinase** (Option C).

### Step 5: Conclusion
The enzyme that is not used for the extraction of a desired gene from a bacterium is **Chitinase**.

---