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Which specific DNA sequence where Eco R1...

Which specific DNA sequence where Eco R1 cuts is
Or Which of the following palindromic sequence is recognized by EcoRI

A

B

C

D

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The correct Answer is:
To solve the question regarding the specific DNA sequence recognized by EcoRI, we can follow these steps: ### Step 1: Understand the concept of palindromic sequences A palindromic sequence in DNA is one that reads the same in both directions (5' to 3' and 3' to 5'). This characteristic is crucial for the recognition by restriction enzymes like EcoRI. **Hint:** Remember that palindromic sequences are symmetrical and can be read the same way in both directions. ### Step 2: Identify the restriction enzyme EcoRI is a restriction enzyme that recognizes specific sequences in DNA. Understanding which sequences are recognized by EcoRI is essential to answer the question. **Hint:** Focus on the function of restriction enzymes and their specific recognition sites. ### Step 3: Determine the specific sequence recognized by EcoRI The specific palindromic sequence recognized by EcoRI is GAATTC. This sequence is crucial as it indicates where EcoRI will cut the DNA. **Hint:** Look for the sequence that is commonly associated with EcoRI in textbooks or reliable biological resources. ### Step 4: Understand the cutting mechanism EcoRI cuts between the G and A in the sequence GAATTC, creating sticky ends. This means that it breaks the phosphodiester bond between these nucleotides, allowing for the formation of complementary sticky ends. **Hint:** Consider how the cutting of DNA by restriction enzymes can facilitate cloning and genetic engineering. ### Step 5: Conclude the answer Based on the information gathered, the palindromic sequence recognized by EcoRI is GAATTC. **Final Answer:** The specific DNA sequence recognized by EcoRI is GAATTC. ### Summary of Steps: 1. Understand palindromic sequences. 2. Identify EcoRI as a restriction enzyme. 3. Determine the specific sequence GAATTC. 4. Understand the cutting mechanism of EcoRI. 5. Conclude with the answer.
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