In anaerobic glycolysis, 2 molecules of inorganic phosphate are used per molecule of glucose consumed. Which of the following enzyme catalyzes the uptake of inorganic phosphate?

Options

A. In anaerobic glycolysis, 2 molecules of inorganic phosphate are used per molecule of glucose consumed. Which of the following enzyme catalyzes the uptake of inorganic phosphate?
B. Hexokinase
C. Phosphofructokinase
D. Glyceraldehyde 3 - phosphate dehydrogenase

Answer

To solve the question regarding which enzyme catalyzes the uptake of inorganic phosphate in anaerobic glycolysis, we can follow these steps:

### Step 1: Understand the Process of Glycolysis
Glycolysis is a metabolic pathway that converts glucose into pyruvate, generating energy in the form of ATP and NADH. It occurs in the cytoplasm and does not require oxygen, hence it is termed anaerobic.

**Hint:** Remember that glycolysis is a series of enzymatic reactions that break down glucose.

### Step 2: Identify the Role of Inorganic Phosphate
In glycolysis, inorganic phosphate (Pi) is used to phosphorylate intermediates, which is essential for the energy yield of the pathway. Specifically, it is involved in the conversion of certain substrates into their phosphorylated forms.

**Hint:** Look for the steps in glycolysis where phosphate groups are added to the substrates.

### Step 3: Review Key Enzymes in Glycolysis
The key enzymes involved in the glycolytic pathway include:
- Hexokinase
- Phosphofructokinase
- Glyceraldehyde 3-phosphate dehydrogenase
- Phosphoglycerate kinase
- Pyruvate kinase

**Hint:** Focus on the enzymes that are directly involved in transferring phosphate groups to substrates.

### Step 4: Identify the Enzyme that Catalyzes the Uptake of Inorganic Phosphate
The enzyme that catalyzes the uptake of inorganic phosphate (Pi) during glycolysis is **Glyceraldehyde 3-phosphate dehydrogenase**. This enzyme facilitates the conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, during which a molecule of inorganic phosphate is added.

**Hint:** Look for the step in glycolysis where NADH is produced, as it is closely associated with the uptake of inorganic phosphate.

### Step 5: Conclusion
Thus, the enzyme that catalyzes the uptake of inorganic phosphate in anaerobic glycolysis is **Glyceraldehyde 3-phosphate dehydrogenase**.

**Final Answer:** Glyceraldehyde 3-phosphate dehydrogenase.