Which of the following is correctly mathced?

Options

A. pbR322-Enzyme
B. Ligase-Molecular glue
C. Agrobacterium-Production of insulin
D. EcoRI-Plamid vector

Answer

To solve the question "Which of the following is correctly matched?", we will analyze each option provided in the question step by step.

### Step 1: Analyze Option 1 - PBR 322
- **Description**: PBR 322 is a plasmid vector used in genetic engineering.
- **Match**: The option states "PBR 322 enzyme".
- **Conclusion**: This is incorrect because PBR 322 is not an enzyme; it is a plasmid vector.

### Step 2: Analyze Option 2 - Ligase
- **Description**: Ligase is an enzyme that facilitates the joining of two nucleotides by forming phosphodiester bonds.
- **Match**: The option states "Ligase molecular glue".
- **Conclusion**: This is correct because ligase is indeed referred to as "molecular glue" due to its function of joining nucleotides together.

### Step 3: Analyze Option 3 - Agrobacterium
- **Description**: Agrobacterium is a genus of bacteria often used in genetic engineering, particularly for plant transformation.
- **Match**: The option states "Agrobacterium production of insulin".
- **Conclusion**: This is incorrect because the production of insulin through recombinant DNA technology typically utilizes E. coli or Saccharomyces, not Agrobacterium.

### Step 4: Analyze Option 4 - Eco R1
- **Description**: Eco R1 is a restriction endonuclease that cuts DNA at specific sites.
- **Match**: The option states "Eco R1 plasmid vector".
- **Conclusion**: This is incorrect because Eco R1 is not a plasmid vector; it is an enzyme used in genetic engineering.

### Final Conclusion
After analyzing all the options, the only correctly matched option is **Option 2: Ligase - Molecular Glue**.