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Which of these is not the feature of a c...

Which of these is not the feature of a cloning vector?
(a) Ori supporting high copy number
(b) Selectable marker
(c) Resistant to the action of restriction enzymes
(d) Presence of cloning site

A

Ori supporting high copy number

B

Selectable marker

C

Resistant to the action of restriction enzymes

D

Presence of cloning site

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question "Which of these is not the feature of a cloning vector?", we need to analyze the options provided and understand the characteristics of cloning vectors. ### Step-by-Step Solution: 1. **Understanding Cloning Vectors**: Cloning vectors are small pieces of DNA that are used to carry foreign DNA fragments (genes of interest) into host cells for replication and expression. They are essential tools in genetic engineering. 2. **Identifying Features of Cloning Vectors**: - **(a) Ori supporting high copy number**: - The origin of replication (ori) is crucial for the replication of the cloning vector within the host cell. A high copy number means that many copies of the vector can be produced, which is a desirable feature. - **(b) Selectable marker**: - Selectable markers are genes that provide a way to identify which cells have successfully taken up the cloning vector. Common examples include antibiotic resistance genes. This is also a necessary feature. - **(c) Resistant to the action of restriction enzymes**: - Cloning vectors must have specific restriction sites where restriction enzymes can cut the DNA. If a vector were resistant to restriction enzymes, it would not be able to be cut and thus would not allow for the insertion of the foreign DNA. Therefore, this is not a feature of cloning vectors. - **(d) Presence of cloning site**: - A cloning site (or multiple cloning site) is where the foreign DNA can be inserted. This is a fundamental feature of any cloning vector. 3. **Evaluating the Options**: - Option (a) is a feature of cloning vectors. - Option (b) is a feature of cloning vectors. - Option (c) is **not** a feature of cloning vectors. - Option (d) is a feature of cloning vectors. 4. **Conclusion**: The option that is not a feature of a cloning vector is **(c) Resistant to the action of restriction enzymes**. ### Final Answer: (c) Resistant to the action of restriction enzymes
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