A plant leaf is found to have bundle sheath cells having large-sized chloroplasts full of strach granules. Which of the following characteristics can be observed in this plant?
I. Stomata open at night.
II. Presence of PEP carboxylase in mesophylls.
III. Presence of Rubis Co in bundle sheath cells.
IV High photorespiration rate in hot summer days.
V. Light reaction and carbon fixation as carbohydrates occur in different cell types.
VI. The carbon assimilation rate is saturated in the early morning on summer days.

Options

A. Only I, III
B. Only II, IV
C. Only II, IV, V
D. Only II, III, V

Answer

To solve the question regarding the characteristics observed in a plant leaf with bundle sheath cells containing large-sized chloroplasts full of starch granules, we will analyze each statement one by one.

### Step-by-Step Solution:

1. **Analyze Statement I: Stomata open at night.**
- In plants with bundle sheath cells and large chloroplasts, such as C4 plants, the stomata typically open during the day for photosynthesis. Therefore, this statement is **incorrect**.

2. **Analyze Statement II: Presence of PEP carboxylase in mesophylls.**
- C4 plants utilize PEP carboxylase to fix carbon dioxide into a four-carbon compound in the mesophyll cells. This statement is **correct**.

3. **Analyze Statement III: Presence of Rubisco in bundle sheath cells.**
- In C4 plants, Rubisco is present in the bundle sheath cells where the Calvin cycle occurs. This statement is **correct**.

4. **Analyze Statement IV: High photorespiration rate in hot summer days.**
- C4 plants are adapted to minimize photorespiration, especially in hot conditions. Therefore, this statement is **incorrect**.

5. **Analyze Statement V: Light reaction and carbon fixation as carbohydrates occur in different cell types.**
- In C4 plants, the light reactions occur in mesophyll cells, while the Calvin cycle (carbon fixation) occurs in bundle sheath cells. This statement is **correct**.

6. **Analyze Statement VI: The carbon assimilation rate is saturated in the early morning on summer days.**
- In C4 plants, carbon assimilation is typically high during the day, not just in the early morning. This statement is **incorrect**.

### Summary of Correct Statements:
- Correct statements are II (Presence of PEP carboxylase in mesophylls), III (Presence of Rubisco in bundle sheath cells), and V (Light reaction and carbon fixation occur in different cell types).

### Final Answer:
The correct characteristics observed in this plant are **II, III, and V**.