Home
Class 12
BIOLOGY
What is insertional inactivation?...

What is insertional inactivation?

A

Inactivation of the host cell.

B

Inactivation of plasmid

C

Inactivation of the gene for the synthesis of the enzyme.

D

Inactivation of ori site

Text Solution

Verified by Experts

The correct Answer is:
C
Promotional Banner

Similar Questions

Explore conceptually related problems

Which of the following enzyme will get inactivated in insertional inactivation

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?

Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour. A) Both statements 1 and 2 are correct B) statement 1 is correct but statement 2 is incorrect C) statement 1 is incorrect but statement 2 is correct D) Both statements 1 and 2 are incorrect

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

Select the incorrect statement wrt insertional inactivation

Assetion: Insertion of recombinant DNA within the coding sequence of beta- galactosidase result n colourless colonies. Reason : Presence of insert results in inactivation of enzyme beta- galactosidase known as insertional inactivation

To which of the following techniques would you associ- ate the phenomenon of insertional inactivation?

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

True and false : In insertional inactivation process alien gene introduced in ori. Sequence.