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Which of the following compound is used ...

Which of the following compound is used in visualisation of DNA fragments in gel electrophoresis?

A

Hexachlorobenzene

B

Silver bromode

C

Ethyl chloride

D

Ethidium bromide

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question "Which of the following compound is used in visualization of DNA fragments in gel electrophoresis?", we can follow these steps: ### Step 1: Understand the Context Gel electrophoresis is a technique used to separate DNA fragments based on their size. After separation, it is important to visualize these fragments to analyze the results. **Hint:** Recall that DNA fragments are not visible to the naked eye after electrophoresis. ### Step 2: Identify the Need for Visualization Since DNA fragments are transparent and dissolve in the buffer used during electrophoresis, we need a method to visualize them. This is typically done using a fluorescent dye. **Hint:** Think about substances that can bind to DNA and emit fluorescence under UV light. ### Step 3: Evaluate the Options The options provided are: - Hexachlorobenzene - Silver chromite - Ethyl chloride - Ethidium bromide **Hint:** Consider which of these compounds is known for its interaction with DNA. ### Step 4: Analyze Each Option 1. **Hexachlorobenzene**: This is a chemical compound that does not have any known application in DNA visualization. 2. **Silver chromite**: This is not used for visualizing DNA in gel electrophoresis. 3. **Ethyl chloride**: This is a solvent and does not interact with DNA for visualization purposes. 4. **Ethidium bromide**: This is a well-known intercalating agent that binds to DNA and fluoresces under UV light. **Hint:** Look for the compound that is specifically mentioned in scientific literature for DNA visualization. ### Step 5: Conclusion Based on the analysis, the correct answer is **Ethidium bromide**. It is widely used in laboratories for the visualization of DNA fragments after gel electrophoresis due to its ability to intercalate with DNA and emit fluorescence when exposed to UV light. **Final Answer:** Ethidium bromide
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