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The vector also have one unique recognit...

The vector also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contains unique recognition sites region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:

A

Ability to separate DNA fragments

B

Flexibility in the choice of restriction enzyme

C

Flexibility in selectable marker

D

Ability of DNA to mutate itself

Text Solution

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The correct Answer is:
To solve the question regarding the function of a polylinker or multiple cloning site (MCS) in vectors used for recombinant DNA (rDNA) technology, we will analyze the provided options step by step. ### Step-by-Step Solution: 1. **Understanding the Concept of Vectors and Polylinkers:** - Vectors are DNA molecules used to carry foreign DNA into a host cell. - A polylinker or multiple cloning site (MCS) is a short segment of DNA within a vector that contains multiple unique recognition sites for restriction enzymes. 2. **Analyzing the Purpose of MCS:** - The primary function of an MCS is to provide multiple unique sites for the insertion of foreign DNA. This allows researchers to choose from various restriction enzymes to cut the vector and insert the desired DNA fragment. 3. **Evaluating the Options:** - **Option A: Ability to separate DNA fragments.** - This is incorrect. The MCS does not separate DNA fragments; it provides sites for inserting them. - **Option B: Flexibility in choice of restriction enzyme.** - This is correct. The presence of multiple restriction sites allows for flexibility in selecting which restriction enzyme to use for cutting the vector. - **Option C: Flexibility in a selectable marker.** - This is incorrect. Selectable markers are separate elements in the vector that help identify successfully transformed cells, not functions of the MCS. - **Option D: Ability of the DNA to mutate itself.** - This is incorrect. The ability of DNA to mutate is not related to the MCS but rather to other elements within the plasmid, such as the origin of replication (ORI). 4. **Conclusion:** - Based on the analysis, the most accurate answer is **Option B: Flexibility in choice of restriction enzyme**. The MCS provides multiple sites for various restriction enzymes, allowing for greater versatility in cloning foreign DNA into the vector. ### Final Answer: **Option B: Flexibility in choice of restriction enzyme.**
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