Read the following statements. Which one of the following is incorrect?

Options

A. GAATTC is the recognition site of EcoRl.
B. Agrobacterium tumefaciens is used for cloning genes in plants
C. In the restriction enzyme Ecori, ''co'' stands for coenzyme.
D. For transformation, micro - particles coated with DNA to be bombarded with gene gun are made up of gold of tungsten.

Answer

To determine which statement is incorrect, we will analyze each statement provided in the question step by step.

### Step 1: Analyze the first statement
**Statement:** "JATT is a recognition site of EcoRI."

- **Explanation:** EcoRI is a restriction endonuclease that recognizes specific palindromic sequences in DNA. The recognition sequence for EcoRI is actually GAATTC, not JATT. Therefore, this statement is incorrect.

### Step 2: Analyze the second statement
**Statement:** "Agrobacterium tumefaciens is used for cloning genes in plants."

- **Explanation:** Agrobacterium tumefaciens is indeed used as a vector for transferring genes into plant cells. This statement is correct.

### Step 3: Analyze the third statement
**Statement:** "In restriction enzyme EcoRI, CO stands for coenzyme."

- **Explanation:** The "Eco" in EcoRI refers to Escherichia coli, the bacterium from which the enzyme was isolated. The "R" stands for the strain, and "I" indicates that it is the first enzyme discovered from that strain. Therefore, this statement is incorrect.

### Step 4: Analyze the fourth statement
**Statement:** "Transformation microparticles coated with DNA bombarded with a gene gun are made up of gold and tungsten."

- **Explanation:** In the gene gun method, microparticles made of gold or tungsten are indeed coated with DNA and then used to transform cells by being bombarded into them. This statement is correct.

### Conclusion
After analyzing all statements, we find that the incorrect statements are:
1. "JATT is a recognition site of EcoRI." (Incorrect)
2. "In restriction enzyme EcoRI, CO stands for coenzyme." (Incorrect)

However, since the question asks for one incorrect statement, the most explicitly incorrect statement is the third one.

### Final Answer
The incorrect statement is: **"In restriction enzyme EcoRI, CO stands for coenzyme."**

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