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Choose the incorrect statement:...

Choose the incorrect statement:

A

Callus is an unorganised mass of cells

B

Growth of callus culture is faster than suspension culture

C

In callus culture, if high auxin to cytokinin ratio is maintained then roots are formed

D

In callus culture, if high cytokinin to auxin ratio is maintained then shoots are formed

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question of identifying the incorrect statement regarding callus and suspension cultures, we can follow these steps: ### Step 1: Understand the Definition of Callus - **Callus** is defined as an unorganized mass of cells that is actively dividing. It is irregular and undifferentiated, meaning the cells do not form structured tissues. **Hint:** Recall the characteristics of callus in plant tissue culture. ### Step 2: Analyze the Callus Culture Process - In callus culture, cell division occurs in the explant (the piece of plant tissue used for culture) to form callus. The medium used typically contains growth regulators, such as auxins (like 2,4-D) and cytokinins (like BAP). **Hint:** Focus on the role of growth regulators in callus formation. ### Step 3: Compare Callus Culture with Suspension Culture - **Suspension culture** consists of single cells or small groups of cells suspended in a liquid medium. This culture method allows for faster growth compared to callus culture due to the constant agitation of the medium. **Hint:** Think about how the physical state of the culture medium affects cell growth. ### Step 4: Evaluate the Statements - The first statement claims that callus is an unorganized mass of cells, which is true. - The second statement suggests that the growth of callus culture is slower than that of suspension culture, which is also true. - The third statement discusses the relationship between auxin and cytokinin ratios in callus culture, which is a correct statement. **Hint:** Identify which statement contradicts the established facts about callus and suspension cultures. ### Step 5: Identify the Incorrect Statement - Since the question asks for the incorrect statement, we need to determine if any of the statements provided are false. After evaluating, we find that all statements are true except for the implication that the growth of callus culture is faster than suspension culture, which is false. **Conclusion:** The incorrect statement is the one that suggests the growth rate of callus culture is faster than that of suspension culture. ### Final Answer - The incorrect statement is: "The growth of callus culture is slower than the suspension culture."
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