In which of the following methods of ART, fertilization will be In-vivo?

Options

A. IVF-ET
B. ZIFT
C. ICSI
D. Al technique

Answer

To determine which method of Assisted Reproductive Technology (ART) involves in vivo fertilization, we will analyze each option provided in the question.

### Step-by-Step Solution:

1. **Understanding In Vivo Fertilization**:
- In vivo fertilization refers to the process where fertilization occurs inside the female body. This means that both the sperm and the egg meet and fuse within the reproductive tract of the female.

2. **Analyzing the First Option - IVF-ET (In Vitro Fertilization - Embryo Transfer)**:
- In IVF-ET, the sperm and egg are collected and fertilized outside the body in a laboratory (in vitro). After fertilization, the embryo is then transferred into the female body. Since fertilization occurs outside the body, this method does not qualify as in vivo fertilization.

3. **Analyzing the Second Option - ZIPT (Zygote Intrafallopian Transfer)**:
- Similar to IVF-ET, in ZIPT, the sperm and egg are also fertilized in the laboratory. The resulting zygote is then transferred to the fallopian tube. Therefore, fertilization occurs in vitro, and this option is also not in vivo.

4. **Analyzing the Third Option - ICSI (Intracytoplasmic Sperm Injection)**:
- In ICSI, a single sperm is injected directly into an egg in a laboratory setting. This is another form of in vitro fertilization, as the fertilization occurs outside the female body. Thus, this option does not represent in vivo fertilization.

5. **Analyzing the Fourth Option - AI (Artificial Insemination)**:
- In AI, sperm is introduced directly into the female's reproductive tract (vagina or uterus) using a medical procedure. The fertilization occurs inside the female body, which qualifies this method as in vivo fertilization.

### Conclusion:
- The method of ART where fertilization occurs in vivo is **Artificial Insemination (AI)**.

### Final Answer:
- **AI (Artificial Insemination)** is the correct answer.