In microsporangium, cells of which layer have more than one nucleus?

Options

A. Epidermis
B. Tapetum
C. Middle layers
D. Endothecium

Answer

To solve the question "In microsporangium, cells of which layer have more than one nucleus?", we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Structure of Microsporangium**:
- The microsporangium is a structure that produces microspores, which develop into pollen grains. It consists of several layers.

2. **Identify the Layers of Microsporangium**:
- The microsporangium typically has four wall layers:
- Epidermis
- Endothesium
- Middle layers
- Tapetum

3. **Analyze Each Layer**:
- **Epidermis**: This is the outermost layer and consists of a single layer of cells that are uninucleated (having one nucleus).
- **Endothesium**: This layer is located just beneath the epidermis and also consists of uninucleated cells.
- **Middle Layers**: These consist of three to four layers of cells, which are also uninucleated.
- **Tapetum**: This is the innermost layer of the microsporangium and surrounds the sporogenous tissue.

4. **Focus on the Tapetum**:
- The tapetum is unique because it is described as syncytial, meaning it has more than one nucleus. This is due to specific processes:
- **Endomitosis**: This process involves DNA replication without cell division, leading to an increase in the number of nuclei within a single cell.
- **Restitution Nuclei**: In this process, during anaphase, the cell divides its genetic material but does not divide the cytoplasm, resulting in multiple nuclei within a single cell surrounded by a common nuclear membrane.

5. **Conclusion**:
- Based on the analysis, the layer of the microsporangium that has more than one nucleus is the **Tapetum**.

### Final Answer:
The cells of the **Tapetum** layer in the microsporangium have more than one nucleus.

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