Which of the following technique is not required for DNA fingerprinting?

Options

A. Polymerase chain reaction
B. ELISA
C. Restriction enzymes
D. DNA - DNA hybridization

Answer

To determine which technique is not required for DNA fingerprinting, let's analyze each option provided:

1. **Polymerase Chain Reaction (PCR)**:
- PCR is a technique used to amplify specific DNA sequences. In DNA fingerprinting, PCR is essential because it allows for the generation of multiple copies of DNA, particularly Short Tandem Repeats (STRs) which are crucial for analysis.
- **Conclusion**: PCR is required for DNA fingerprinting.

2. **Enzyme Linked Immunosorbent Assay (ELISA)**:
- ELISA is primarily a biochemical technique used to detect the presence of antibodies or antigens in a sample. It is commonly used in medical diagnostics but does not play a role in DNA fingerprinting.
- **Conclusion**: ELISA is not required for DNA fingerprinting.

3. **Restriction Enzymes**:
- Restriction enzymes are proteins that cut DNA at specific sequences. They are used in DNA fingerprinting to analyze DNA fragments by cutting them into smaller pieces for comparison.
- **Conclusion**: Restriction enzymes are required for DNA fingerprinting.

4. **DNA-DNA Hybridization**:
- This technique measures the genetic similarity between DNA sequences. It can be useful in comparing DNA samples in fingerprinting to determine relatedness or genetic similarity.
- **Conclusion**: DNA-DNA hybridization is required for DNA fingerprinting.

Based on the analysis, the technique that is not required for DNA fingerprinting is **Enzyme Linked Immunosorbent Assay (ELISA)**.

### Final Answer:
The technique that is not required for DNA fingerprinting is **ELISA**.