Which enzymes are not used for isolation of the DNA?
I. Cellulose
II. Lysozyme
III. Dnase
IV. Lyase

Options

A. I, III
B. II, III
C. III, IV
D. IV, II

Answer

To determine which enzymes are not used for the isolation of DNA, we will analyze each option provided:

1. **Cellulase**:
- Cellulase is an enzyme that breaks down cellulose, which is a major component of plant cell walls. While cellulase can be involved in the process of breaking down plant material to access DNA, it is not directly used for isolating DNA itself. Therefore, it is not primarily associated with DNA isolation.

2. **Lysozyme**:
- Lysozyme is an enzyme that degrades the cell walls of bacteria and yeast. It is commonly used in microbial DNA isolation protocols to lyse cells and release DNA. Thus, lysozyme is indeed used for DNA isolation.

3. **DNase**:
- DNase is an enzyme that degrades DNA. It is typically used in applications where the removal of DNA is necessary, such as RNA isolation. Therefore, DNase is not used for isolating DNA, as it would degrade the DNA instead.

4. **Lyase**:
- Lyases are enzymes that catalyze the addition or removal of groups to form double bonds or vice versa. They are not typically involved in the process of DNA isolation. Thus, lyase is not used for isolating DNA.

Now, summarizing the findings:
- **Cellulase**: Not primarily used for DNA isolation.
- **Lysozyme**: Used for DNA isolation.
- **DNase**: Not used for DNA isolation.
- **Lyase**: Not used for DNA isolation.

Based on this analysis, the enzymes that are not used for the isolation of DNA are **Cellulase (I)**, **DNase (III)**, and **Lyase (IV)**.

Thus, the correct answer is **I, III, and IV**.