Statement - 1 : Biolistics method of gene transfer is an example of direct gene transfer.
Statement - 2 : In biolistics method , pBR322 is used.

Options

A. Statement-1 is true, Statement-2 is true, Statement-2 is not the correct explanation of Statement-1.
B. Statement-1 is false, Statement-2 is true.
C. Statement-1 is true, Statement-2 is false.
D. Statement-1 is true, Statement-2 is true, Statement-2 is the correct explanation of Statement-1.

Answer

To analyze the given statements about the biolistics method of gene transfer, we will break down the information provided in each statement and evaluate their validity step by step.

### Step 1: Evaluate Statement 1
**Statement 1:** Biolistics method of gene transfer is an example of direct gene transfer.

- **Explanation:** The biolistics method, also known as the gene gun method, involves coating DNA fragments onto small particles (usually gold or tungsten) and then physically bombarding these particles into the target cells. This process allows for the direct introduction of DNA into the cells without the need for a vector. Therefore, this method is indeed classified as a direct gene transfer method.

- **Conclusion:** Statement 1 is **True**.

### Step 2: Evaluate Statement 2
**Statement 2:** In biolistics method, pBR322 is used.

- **Explanation:** pBR322 is a plasmid vector commonly used in molecular cloning. However, in the biolistics method, the DNA is not introduced using a vector like pBR322. Instead, the DNA is directly coated onto the particles and delivered into the cells. Therefore, the use of pBR322 or any other vector is not applicable in the biolistics method.

- **Conclusion:** Statement 2 is **False**.

### Final Conclusion
Based on the evaluations:
- Statement 1 is True.
- Statement 2 is False.

Thus, the correct answer is that Statement 1 is true and Statement 2 is false.

### Answer:
**Option C:** Statement 1 is true and Statement 2 is false.

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