Mendel's law of segregation was based on the separation of alleles in the garden pea during:

Options

A. Pollination
B. Embryonic development
C. Seed formation
D. Gamete formation

Answer

To solve the question regarding Mendel's law of segregation, we can follow these steps:

### Step 1: Understand Mendel's Law of Segregation
Mendel's law of segregation states that during the formation of gametes, the two alleles for a trait separate from each other so that each gamete carries only one allele for each trait.

**Hint:** Think about how traits are inherited and the role of alleles.

### Step 2: Identify the Context of the Question
The question asks during which process the separation of alleles occurs in the garden pea. The options provided are:
1. Pollination
2. Embryonic development
3. Seed formation
4. Gamete formation

**Hint:** Focus on the processes involved in the formation of gametes.

### Step 3: Analyze Each Option
- **Pollination:** This is the transfer of pollen from the male part to the female part of a flower. It does not involve the separation of alleles.
- **Embryonic development:** This occurs after fertilization and involves the growth of the zygote into an embryo. Alleles are already combined at this stage.
- **Seed formation:** This occurs after fertilization and involves the development of seeds from the fertilized ovule. The alleles have already been combined.
- **Gamete formation:** This is the process where gametes (sperm and egg) are produced, and it is during this process that the two alleles segregate.

**Hint:** Think about when alleles are separated to ensure that each gamete has only one allele.

### Step 4: Conclusion
Based on the analysis, the correct answer is **gamete formation**. This is the process during which the alleles separate, allowing for the inheritance of traits according to Mendel's law of segregation.

**Final Answer:** Gamete formation (Option 4).