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Probe used in molecular diagnosis of dis...

Probe used in molecular diagnosis of diseases can be

A

a single stranded or double stranded DNA molecule tagged with a radioactive molecule

B

a single stranded or double stranded RNA molecule tagged with a radioactive molecule

C

a single stranded DNA or RNA molecule tagged with a radioactive molecule

D

a double stranded DNA or RNA molecule tagged with a radioactive molecule

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The correct Answer is:
To solve the question regarding probes used in molecular diagnosis of diseases, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding Probes**: - Probes are short sequences of nucleic acids (DNA or RNA) that are used to detect the presence of complementary sequences in a sample. They play a crucial role in molecular diagnostics. 2. **Types of Probes**: - Probes can be either single-stranded or double-stranded. However, for the purpose of molecular diagnosis, single-stranded probes are more commonly used because they can easily hybridize with their complementary sequences. 3. **Labeling of Probes**: - Probes are often tagged with a radioactive molecule or a fluorescent marker to facilitate detection. This tagging allows researchers to visualize or quantify the binding of the probe to the target sequence. 4. **Analyzing the Options**: - The question provides four options: 1. Single-stranded or double-stranded DNA molecule tagged with a radioactive molecule. 2. Single-stranded or double-stranded RNA molecule tagged with a radioactive molecule. 3. Single-stranded DNA or RNA molecule tagged with a radioactive molecule. 4. Double-stranded DNA or RNA molecule tagged with a radioactive molecule. - Among these, the most suitable option for molecular diagnosis is the one that specifies single-stranded DNA or RNA. 5. **Identifying the Correct Answer**: - The correct answer is option 3: "a single-stranded DNA or RNA molecule tagged with a radioactive molecule." This is because single-stranded probes are effective for hybridization with complementary sequences in the target DNA or RNA. ### Final Answer: The probe used in molecular diagnosis of diseases can be a **single-stranded DNA or RNA molecule tagged with a radioactive molecule**. ---
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Assertion: A ds DNA, tagged with a radioactive molecule (probe) is used in medical diagnosis Reason :It is complementary to mutated gene