Diabetes mellitus may be treated using insulin from genetic engineering . Where is this insulin produced ?

Options

A. Bacterial cytoplasm
B. Bacterial nucleus
C. Human liver
D. Human pancreas

Answer

To answer the question about where insulin produced through genetic engineering is made, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Context**: The question relates to the treatment of diabetes mellitus using insulin produced via genetic engineering techniques.

2. **Identifying the Source of Insulin**: Insulin is a hormone that is naturally produced in the human pancreas. However, in the context of genetic engineering, we are looking for where the artificially produced insulin is synthesized.

3. **Genetic Engineering Process**: The process begins by isolating the gene that codes for insulin from human pancreatic tissue. This gene is then inserted into bacteria (commonly E. coli).

4. **Bacterial Multiplication**: Once the insulin gene is introduced into the bacteria, these bacteria are allowed to multiply. As they replicate, they also produce insulin because they now contain the human insulin gene.

5. **Location of Insulin Production**: The production of insulin occurs within the bacteria. Specifically, it takes place in the bacterial cytoplasm, where the cellular machinery can translate the inserted gene into the insulin protein.

6. **Conclusion**: Given the options provided (bacterial cytoplasm, bacterial nucleus, human liver, or human pancreas), the correct answer is that insulin is produced in the **bacterial cytoplasm**.

### Final Answer:
**Insulin is produced in the bacterial cytoplasm.**

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