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From the following, the statement /s tha...

From the following, the statement /s that will help to determine the specificity of an enzyme is /are:
I. The bonding between R groups of amino acids of the polypeptide
II. The optimum pH of the enzyme
III. The peptide bonds between amino acids of the polypeptide
IV. The shape of the substrate molecule

A

I,II,III and IV

B

I and III only

C

I only

D

II,III and IV only

Text Solution

AI Generated Solution

The correct Answer is:
To determine the specificity of an enzyme, we need to analyze each of the statements provided in the question. 1. **Statement I: The bonding between R groups of amino acids of the polypeptide.** - This statement is correct. The specificity of an enzyme is largely determined by the structure of its active site, which is formed by the folding of the polypeptide chain. The R groups (side chains) of the amino acids in the active site interact with the substrate, allowing the enzyme to bind specifically to its substrate. 2. **Statement II: The optimum pH of the enzyme.** - This statement is incorrect. While the optimum pH can affect the activity of an enzyme (how fast it works), it does not determine the specificity of the enzyme. Specificity is about which substrate the enzyme can act upon, not how well it works under different pH conditions. 3. **Statement III: The peptide bonds between amino acids of the polypeptide.** - This statement is also incorrect. Peptide bonds link amino acids together to form the polypeptide chain, but they do not influence the specificity of the enzyme. The specificity is determined by the R groups and the overall three-dimensional shape of the active site. 4. **Statement IV: The shape of the substrate molecule.** - This statement is misleading. While the shape of the substrate is important for the enzyme-substrate interaction, specificity is determined by the enzyme's active site structure. The enzyme must have a complementary shape to the substrate for binding to occur, but this statement does not directly address the enzyme's specificity. **Conclusion:** The only statement that directly helps to determine the specificity of an enzyme is Statement I. Therefore, the correct answer is that only Statement I is relevant for determining enzyme specificity. ### Summary of the Solution: - **Correct Statement:** I (The bonding between R groups of amino acids of the polypeptide) - **Incorrect Statements:** II, III, IV
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