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EcoRI always cuts DNA molecules at a par...

EcoRI always cuts DNA molecules at a particluar point by recognizing a specific restriction sequence, the sticky ends fromed after digestion have the sequence

A

AATT

B

TCCCA

C

AACTT

D

TTCAC

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To solve the question regarding the EcoRI restriction enzyme and the sticky ends formed after digestion, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding EcoRI**: EcoRI is a restriction enzyme derived from the bacterium Escherichia coli. It recognizes specific sequences in DNA and cuts at those sites. 2. **Identifying the Recognition Sequence**: The specific recognition sequence for EcoRI is "GAATTC". This means that EcoRI will bind to this sequence in the DNA. 3. **Cutting the DNA**: EcoRI does not cut the DNA in the middle of the recognition sequence. Instead, it cuts between the G and the A on one strand and between the C and the T on the complementary strand. This results in the formation of "sticky ends". 4. **Resulting Sticky Ends**: After EcoRI cuts the DNA at the GAATTC sequence, the sticky ends that remain will be: - From the top strand: AATTC - From the bottom strand: TTAA 5. **Final Sticky End Sequence**: The sticky ends that are left after the digestion will be "AATT". This is because the enzyme cuts the DNA in such a way that the overhanging ends are AATC on one side and TTAA on the other, but when we refer to the sticky end, we typically denote the sequence that remains unpaired, which is "AATT". 6. **Conclusion**: Therefore, the sequence of the sticky ends formed after EcoRI digestion is "AATT".
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