Which of the following show a correct pair with respect to pBR322 ?

Options

A. ori - Hind III
B. `tet^R` -BamH, Sal I
C. `amp^R` - Pst , Pvu II
D. rop - Pvu I

Answer

To solve the question regarding the correct pair with respect to pBR322, let's analyze the options step by step:

### Step 1: Understanding pBR322
pBR322 is a plasmid that contains genes for antibiotic resistance (ampicillin and tetracycline) and specific restriction sites for cloning purposes. It is important to identify the correct associations between the components of pBR322.

### Step 2: Analyzing the Options
1. **First Option**: "ori is hind III"
- **Analysis**: The origin of replication (ori) in pBR322 is not associated with the Hind III restriction site. Therefore, this option is incorrect.

2. **Second Option**: "tetracycline resistance is bam HI and sal I"
- **Analysis**: Tetracycline resistance in pBR322 is indeed associated with the Bam HI and Sal I restriction sites. This option is correct.

3. **Third Option**: "ampicillin resistance is pvu I and pst I"
- **Analysis**: The ampicillin resistance gene is associated with the Pst I site, but it is incorrectly stated as Pvu II in this option. Therefore, this option is incorrect.

4. **Fourth Option**: "rob is pvu I"
- **Analysis**: The rob gene is a protein-coding gene, but it is incorrectly stated as associated with Pvu I. The correct association is with Pvu II. Therefore, this option is incorrect.

### Conclusion
After analyzing all the options, the only correct pair with respect to pBR322 is the **second option**: "tetracycline resistance is bam HI and sal I".

### Final Answer
The correct answer is **Option 2**.

---