Which of the following pairs is incorrect matched ?

Options

A. Genetic code - unambiguous
B. Okazaki fragments - Splicing
C. RNA polymerase - RNA primer
D. Restriction enzymes - Genetic engineering

Answer

To solve the question "Which of the following pairs is incorrectly matched?", we will analyze each option provided in the question systematically.

### Step 1: Analyze the first pair - Genetic Code and Unambiguous
- **Explanation**: The genetic code is indeed unambiguous. This means that each codon (a sequence of three nucleotides) corresponds to only one specific amino acid. Therefore, this pair is correctly matched.

### Step 2: Analyze the second pair - Okazaki Fragments and Splicing
- **Explanation**: Okazaki fragments are short sequences of DNA nucleotides that are synthesized during DNA replication. They are formed on the lagging strand and are later joined together by the enzyme DNA ligase. Splicing, on the other hand, refers to the process of removing introns from pre-mRNA to produce mature mRNA. Since Okazaki fragments are not related to splicing, this pair is incorrectly matched.

### Step 3: Analyze the third pair - RNA Polymerase and RNA Primer
- **Explanation**: RNA polymerase is the enzyme responsible for synthesizing RNA from a DNA template. RNA primers are short RNA sequences that provide a starting point for DNA synthesis during replication. While RNA polymerase works with RNA primers, they are not directly matched in the context of their primary functions. However, this pair is generally considered correct in the context of replication.

### Step 4: Analyze the fourth pair - Restriction Enzyme and Genetic Engineering
- **Explanation**: Restriction enzymes are crucial tools in genetic engineering as they cut DNA at specific sequences, allowing scientists to manipulate DNA for various purposes. This pair is correctly matched.

### Conclusion
After analyzing all the pairs, the only incorrect match is the second pair: Okazaki Fragments and Splicing.

### Final Answer
The incorrectly matched pair is: **Okazaki Fragments and Splicing**.

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