During glycolysis in cytoplasm, the decarboxylation reactions

Options

A. are not observed
B. occurs twice
C. occurs once
D. occurs in all the ten steps

Answer

### Step-by-Step Solution:

1. **Understanding Glycolysis**: Glycolysis is the metabolic pathway that converts glucose into pyruvate, occurring in the cytoplasm of the cell. It consists of a series of enzymatic reactions.

2. **Identifying Decarboxylation**: Decarboxylation is a chemical reaction that involves the removal of a carboxyl group (-COOH) from a molecule, releasing carbon dioxide (CO2).

3. **Location of Decarboxylation**: In the context of glycolysis, it is important to note that decarboxylation does not occur during this pathway. Instead, decarboxylation occurs later in the Krebs cycle (citric acid cycle) when pyruvate is converted to acetyl-CoA.

4. **Link Reaction**: The conversion of pyruvate to acetyl-CoA involves a link reaction that occurs after glycolysis. This reaction is catalyzed by the enzyme pyruvate dehydrogenase and involves both dehydrogenation and decarboxylation.

5. **Conclusion**: Since glycolysis itself does not involve any decarboxylation reactions, the correct answer to the question is that decarboxylation reactions are "not observed" during glycolysis.

### Final Answer:
**The decarboxylation reactions during glycolysis in the cytoplasm are not observed.**

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