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In genetic engineering, after the creati...

In genetic engineering, after the creation of recombinant DNA, it has to be transferred into the host using a vector. Which of the following agents can be used as a vector?
A. pBR322
B. E.coli
C. Transposons
D.Mosquito
E. A disarmed Ti plasmid

A

A, C, and E

B

A , B , and E

C

A , B , and C

D

A, B, C , D and E

Text Solution

AI Generated Solution

The correct Answer is:
To answer the question regarding which agents can be used as vectors in genetic engineering, we need to analyze each option based on the definition and characteristics of a vector. ### Step-by-Step Solution: 1. **Understanding the Concept of a Vector**: - A vector is a DNA molecule that can carry foreign DNA into a host cell. - It should have the ability to replicate independently within the host and often contains selectable markers. 2. **Analyzing Option A: pBR322**: - pBR322 is a well-known plasmid vector used in genetic engineering. - It has its own origin of replication (ORI) and contains antibiotic resistance genes (ampicillin and tetracycline resistance). - **Conclusion**: pBR322 can be used as a vector. (Correct) 3. **Analyzing Option B: E. coli**: - E. coli is a type of bacteria that is commonly used as a host for cloning vectors. - It is not a vector itself; rather, it serves as a host cell for the replication of vectors. - **Conclusion**: E. coli cannot be used as a vector. (Incorrect) 4. **Analyzing Option C: Transposons**: - Transposons are DNA sequences that can change their position within the genome and can replicate independently. - They can integrate into the host genome, making them suitable as vectors. - **Conclusion**: Transposons can be used as vectors. (Correct) 5. **Analyzing Option D: Mosquito**: - Mosquitoes are living organisms that can carry pathogens but do not integrate foreign DNA into their genome. - They are not suitable as vectors in the context of genetic engineering. - **Conclusion**: Mosquito cannot be used as a vector. (Incorrect) 6. **Analyzing Option E: A disarmed Ti plasmid**: - Ti plasmids are derived from Agrobacterium tumefaciens and are used in plant genetic engineering. - A "disarmed" Ti plasmid means it has been modified to remove its pathogenicity, allowing it to be used safely as a vector. - **Conclusion**: A disarmed Ti plasmid can be used as a vector. (Correct) ### Final Answer: The agents that can be used as vectors in genetic engineering from the given options are: - A. pBR322 - C. Transposons - E. A disarmed Ti plasmid
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