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A set of 6 test tube having same tempera...

A set of 6 test tube having same temperature but different pH were setup to conduct an experiment . To each of the tubes, starch and amylase were added in a mix of sterile water . The concentration was kept the same . The following table notes the reaction completion time for each of the six tubes.
`{:(PH,1,3,5,7,9,11),("Time taken to complete in seconds (s).",94,64,32,5,35,66):}`
Using the above data , determine the pH where amylase has shown highest rate of activity.

A

pH 1

B

pH 5

C

pH 7

D

pH 11

Text Solution

AI Generated Solution

The correct Answer is:
To determine the pH where amylase has shown the highest rate of activity based on the provided data, we can follow these steps: ### Step 1: Understand the Data We have a set of 6 test tubes with varying pH levels and their corresponding reaction completion times for amylase activity. The pH levels and times are as follows: - pH 1: 94 seconds - pH 3: 64 seconds - pH 5: 32 seconds - pH 7: 5 seconds - pH 9: 35 seconds - pH 11: 66 seconds ### Step 2: Analyze the Reaction Completion Times From the data, we can see that the time taken for the reaction to complete decreases as the pH approaches 7, which is the neutral pH. The reaction time is the shortest (5 seconds) at pH 7, indicating the highest rate of amylase activity. ### Step 3: Identify the Optimum pH The optimum pH for enzyme activity is typically where the reaction time is the shortest. In this case, the reaction time is shortest at pH 7. Therefore, we conclude that amylase shows the highest rate of activity at this pH. ### Step 4: Conclusion Based on the analysis, the pH where amylase has shown the highest rate of activity is **pH 7**. ### Final Answer The pH where amylase has shown the highest rate of activity is **pH 7**. ---
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