Read the following statement regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option.
I. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
II. Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector.
III. The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography.
IV. A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection.
`{:(,I,II,III,IV),(1,"False","False","True","True"),(2,"True","True","False","False"),(3,"True","True","True","True"),(4,"False","False","True","False"):}`

Options

A. 1
B. 2
C. 3
D. 4

Answer

To solve the question regarding DNA recombinant technology, we will analyze each statement one by one to determine whether they are true or false.

### Step-by-Step Solution:

1. **Statement I**: "Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants."
- **Analysis**: Selectable markers are indeed used in recombinant DNA technology to identify which cells have taken up the plasmid (transformants) and to eliminate those that have not (non-transformants). For example, antibiotic resistance genes can be used as selectable markers.
- **Conclusion**: This statement is **True**.

2. **Statement II**: "Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector."
- **Analysis**: Plasmids are small circular DNA molecules that can replicate independently of the chromosomal DNA within bacterial cells. This characteristic makes them excellent vectors for cloning.
- **Conclusion**: This statement is **True**.

3. **Statement III**: "The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography."
- **Analysis**: Thin-layer chromatography (TLC) is typically used for separating small molecules and pigments, not DNA fragments. The correct technique for separating DNA fragments is gel electrophoresis.
- **Conclusion**: This statement is **False**.

4. **Statement IV**: "A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection."
- **Analysis**: The technique described is known as the "gene gun" or biolistic method, not micro-injection. Micro-injection refers to the direct injection of DNA into the nucleus of a cell.
- **Conclusion**: This statement is **False**.

### Summary of Statements:
- I: True
- II: True
- III: False
- IV: False

### Final Answer:
The correct option is **(2)**: "True, True, False, False".

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