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pBR322 has restriction sites for many re...

pBR322 has restriction sites for many restriction endonucleases. Which of these enzymes acting on pBR322 is not correctly matched with its source ?
I. Pvul : Providencia vulgaris.
II. Clal: caryophanon latum.
III. Sall : Streptomyces albus
IV. Pstl: Proteus stuarti

A

I and II

B

II and IV

C

I and IV

D

II and III

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question regarding the restriction endonucleases and their sources in pBR322, we will analyze each option step by step. ### Step 1: Analyze the first option (Pvul) - **Enzyme**: Pvul - **Source**: Providencia vulgaris - **Correct Source**: The correct source of Pvul is actually **Proteus vulgaris**. - **Conclusion**: This option is **not correctly matched**. ### Step 2: Analyze the second option (Clal) - **Enzyme**: Clal - **Source**: Caryophanon latum - **Correct Source**: Clal is correctly sourced from **Caryophanon latum**. - **Conclusion**: This option is **correctly matched**. ### Step 3: Analyze the third option (Sall) - **Enzyme**: Sall - **Source**: Streptomyces albus - **Correct Source**: Sall is correctly sourced from **Streptomyces albus**. - **Conclusion**: This option is **correctly matched**. ### Step 4: Analyze the fourth option (Pstl) - **Enzyme**: Pstl - **Source**: Proteus stuarti - **Correct Source**: The correct source of Pstl is **Providencia stuartii**. - **Conclusion**: This option is **not correctly matched**. ### Final Conclusion: The enzymes that are not correctly matched with their sources are: - **I. Pvul: Providencia vulgaris** (should be Proteus vulgaris) - **IV. Pstl: Proteus stuarti** (should be Providencia stuartii) Thus, the answer to the question is that options I and IV are not correctly matched.
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