In the Avery, MacLoed , and McCarthy experiment, which of the following enzymes did not affect the process of transformation ?

Options

A. Protease
B. RNase
C. DNase
D. Both (A) and (B)

Answer

To solve the question regarding the Avery, MacLeod, and McCarty experiment and which enzymes did not affect the process of transformation, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Avery, MacLeod, and McCarty Experiment**:
- This experiment was crucial in demonstrating that DNA is the genetic material responsible for bacterial transformation. It showed that the transforming principle was DNA, not protein or RNA.

2. **Identify the Enzymes Used in the Experiment**:
- The experiment utilized three types of enzymes:
- **Protease**: An enzyme that digests proteins.
- **RNase**: An enzyme that digests RNA.
- **DNase**: An enzyme that digests DNA.

3. **Analyze the Effect of Each Enzyme on Transformation**:
- The experiment found that when **DNase** was used, it inhibited the transformation process, indicating that DNA was necessary for transformation.
- On the other hand, both **protease** and **RNase** did not affect the transformation process. This means that the presence of proteins and RNA did not influence the ability of the bacteria to undergo transformation.

4. **Conclude Which Enzymes Did Not Affect Transformation**:
- Since both protease and RNase did not affect the transformation, the correct answer to the question is that both A (protease) and B (RNase) did not affect the process of transformation.

### Final Answer:
Both A and B (protease and RNase) did not affect the process of transformation in the Avery, MacLeod, and McCarty experiment.

---