Where will be the human insulin found in the genetically altered prokaryote ?

Options

A. Genetically engineered bacterial cytoplasm
B. Genetically engineered bacterial nucleus
C. Human liver having the modified bacteria
D. Human pancreas having the modified bacteria

Answer

To determine where human insulin will be found in genetically altered prokaryotes, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Source of Insulin**: Human insulin is produced from a segment of DNA that codes for insulin, which is naturally found in the human pancreas.

2. **Extraction of Insulin Gene**: The specific segment of DNA that codes for insulin is extracted from the human genome. This segment is crucial for the production of insulin.

3. **Insertion into Plasmid**: The extracted DNA segment is then inserted into a plasmid, which is a small, circular piece of DNA commonly found in bacteria. This plasmid acts as a vector to carry the insulin gene into the bacterial cell.

4. **Transformation of Bacteria**: The plasmid containing the human insulin gene is introduced into a bacterial cell (a prokaryote). This process is known as transformation, where the bacteria take up the plasmid.

5. **Recombination and Production**: Once inside the bacterial cell, the plasmid integrates into the bacterial DNA, allowing the bacteria to express the human insulin gene. The bacteria then start producing human insulin as they replicate.

6. **Location of Insulin Production**: The production of human insulin occurs in the cytoplasm of the genetically altered bacteria. Therefore, the human insulin will be found in the cytoplasm of the genetically engineered prokaryote.

### Conclusion:
The correct answer is that human insulin will be found in the **genetically engineered bacterial cytoplasm**.