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In recombinant DNA technology before the...

In recombinant DNA technology before the use of restriction endonuclease , we have to isolated and precipitate the genetic material (DNA) . For this purpose we use following different enzyme / chemical :
(a) Ethidium bromide
(b) RNA polymerase
(c) T4 ligase
(d) Chilled Ethanol

A

Ethidium bromide

B

RNA polymerase

C

`T_4` ligase

D

Chilled Ethanol

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question regarding the enzymes and chemicals used in recombinant DNA technology before the use of restriction endonucleases, we will analyze each option provided: 1. **Understanding the Process**: In recombinant DNA technology, the first steps involve isolating and precipitating the genetic material (DNA) from cells. This is essential before any cutting or manipulation of DNA can occur. 2. **Analyzing the Options**: - **(a) Ethidium bromide**: This chemical is primarily used in agarose gel electrophoresis to stain DNA, allowing visualization. It is not used for isolating or precipitating DNA. Therefore, this option is incorrect. - **(b) RNA polymerase**: This enzyme is involved in the transcription process, where RNA is synthesized from a DNA template. It does not play a role in isolating or precipitating DNA. Thus, this option is also incorrect. - **(c) T4 ligase**: T4 ligase is an enzyme used to join DNA fragments together after they have been cut by restriction endonucleases. It is not used in the initial isolation or precipitation of DNA. Hence, this option is incorrect as well. - **(d) Chilled Ethanol**: Chilled ethanol is commonly used to precipitate DNA after it has been isolated from cells. It helps in the aggregation of DNA, making it visible and easier to collect. This is the correct option. 3. **Conclusion**: The only correct choice for the chemical used to isolate and precipitate DNA before the use of restriction endonucleases is **(d) Chilled Ethanol**. ### Summary of the Steps: 1. Identify the purpose of the question: isolation and precipitation of DNA. 2. Evaluate each option based on its role in recombinant DNA technology. 3. Determine that chilled ethanol is the only correct answer for the precipitation of DNA.
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