A gram (+Ve) bacteria differs from a Gram (–ve) bacteria because of

Options

A. Cell membrane
B. Cytoplasm
C. Flagellar
D. Cell wall

Answer

To solve the question regarding the differences between Gram-positive and Gram-negative bacteria, we can break it down into the following steps:

### Step-by-Step Solution:

1. **Understanding Gram Staining**:
- Gram staining is a method used to classify bacteria into two groups based on their cell wall structure.

2. **Cell Wall Composition**:
- **Gram-positive bacteria**: They have a thick cell wall composed of multiple layers of peptidoglycan. This thick layer retains the crystal violet stain used in Gram staining, causing them to appear purple.
- **Gram-negative bacteria**: They possess a thinner layer of peptidoglycan, which is located between the inner cell membrane and an outer membrane. The outer membrane contains lipopolysaccharides (LPS). This structure does not retain the crystal violet stain and instead takes up the counterstain (safranin), appearing pink.

3. **Outer Membrane**:
- Gram-negative bacteria have an additional outer membrane that is absent in Gram-positive bacteria. This outer membrane contributes to the bacteria's overall structure and can affect its permeability and resistance to antibiotics.

4. **Conclusion**:
- The primary difference between Gram-positive and Gram-negative bacteria lies in the structure and composition of their cell walls. Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane.

### Final Answer:
Gram-positive bacteria differ from Gram-negative bacteria primarily due to the structure of their cell walls, with Gram-positive bacteria having a thick peptidoglycan layer and Gram-negative bacteria having a thin peptidoglycan layer along with an outer membrane.