Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium.
(i) Transform bacteria with recombinant
(ii) Cut the plasmid DNA using restriction enzyme .
(iii) Extract plasmid DNA to - DNA fragments DNA
(iv) Bind the plasmid DNA to non-plasmid DNA fragments .
(v) Use ligase to seal plasmid DNA to non- plasmid DNA
Arrange the above steps in the right sequence and select the CORRECT option

Options

A. iv, v, ii, iii , iv
B. iii , ii , iv ,v, i
C. iii, iv , v, i, ii
D. ii, iii, v, iv ,i

Answer

To solve the problem of arranging the steps for slicing foreign DNA into a plasmid and inserting the plasmid into a bacterium, we need to follow the logical sequence of molecular cloning procedures. Here’s the step-by-step solution:

### Step-by-Step Solution:

1. **Extract Plasmid DNA**: The first step is to extract the plasmid DNA from the bacterial cells. This provides the vector that will carry the foreign DNA.

2. **Cut the Plasmid DNA Using Restriction Enzyme**: The next step involves using a restriction enzyme to cut the plasmid DNA at specific sites. This creates openings in the plasmid where the foreign DNA can be inserted.

3. **Bind the Plasmid DNA to Non-Plasmid DNA Fragments**: After cutting the plasmid, the foreign DNA (non-plasmid DNA) is introduced and binds to the cut ends of the plasmid DNA.

4. **Use Ligase to Seal Plasmid DNA to Non-Plasmid DNA**: The enzyme ligase is then used to seal the nicks in the sugar-phosphate backbone of the DNA, effectively joining the plasmid and the foreign DNA together to form a recombinant DNA molecule.

5. **Transform Bacteria with Recombinant DNA**: The final step is to introduce the recombinant plasmid into bacterial cells through a process called transformation. This allows the bacteria to take up the plasmid and replicate it.

### Correct Sequence:
The correct sequence of steps is:
1. Extract plasmid DNA
2. Cut the plasmid DNA using restriction enzyme
3. Bind the plasmid DNA to non-plasmid DNA fragments
4. Use ligase to seal plasmid DNA to non-plasmid DNA
5. Transform bacteria with recombinant DNA

### Final Answer:
The correct option that matches this sequence is **Option 2**.