With respect to RFLP , arrange the following sentences in order .
I. Agarose gel electrophoresis
II. Isolation on DNA
III . Cutting with Restriction Enzyme
IV. PCR

Options

A. I, II, III, IV
B. II, I, IV, III
C. II, III, IV, I
D. II, IV, III, I

Answer

To arrange the sentences in the correct order with respect to RFLP (Restriction Fragment Length Polymorphism), we will analyze the steps involved in the RFLP process. Here’s the step-by-step solution:

1. **Isolation of DNA (II)**: The first step in the RFLP process is to isolate DNA from the cells. This is crucial as we need a sample of DNA to work with.

2. **PCR (IV)**: The next step is to amplify the isolated DNA using Polymerase Chain Reaction (PCR). This step increases the quantity of DNA, making it easier to work with in subsequent steps.

3. **Cutting with Restriction Enzyme (III)**: After amplification, the DNA is then cut into fragments using restriction enzymes. These enzymes recognize specific sequences in the DNA and cut it at those sites, resulting in fragments of varying lengths.

4. **Agarose Gel Electrophoresis (I)**: Finally, the DNA fragments are separated using agarose gel electrophoresis. This technique allows us to visualize the different lengths of DNA fragments, which can be analyzed for polymorphisms.

Putting it all together, the correct order is:
- II (Isolation of DNA)
- IV (PCR)
- III (Cutting with Restriction Enzyme)
- I (Agarose Gel Electrophoresis)

### Final Arrangement:
II → IV → III → I