In Ell Lily"s method of inulin production , which of the following is correct ?

Options

A. A peptide and B peptide were produced from the same host cell
B. A peptide and B peptide were produced from different host cells.
C. A peptide and B peptide C peptide were produced from the same host cell
D. A peptide and B peptide C peptide were produced from different host cell s.

Answer

**Step-by-Step Solution:**

1. **Understanding L. Lilly's Method:**
- L. Lilly is an American company known for producing the first synthetic insulin using genetic engineering, which was launched in 1983.

2. **Peptide Composition of Insulin:**
- Insulin consists of two main peptides: A peptide and B peptide. These peptides are crucial for the formation of functional insulin.

3. **Isolation and Preparation:**
- In L. Lilly's method, the A and B peptides are isolated and prepared. Each peptide is introduced into a plasmid.

4. **Use of Host Cells:**
- The plasmids containing the A and B peptides are then inserted into host cells, specifically E. coli, which is a common host for genetic engineering.

5. **Production of Peptides:**
- Both A and B peptides are produced from different E. coli host cells. This means that although they are both produced in E. coli, they are not produced from the same host cell.

6. **Purification and Combination:**
- After production, the peptides are purified and then combined to form recombinant insulin through disulfide linkages.

7. **Analyzing the Options:**
- Option A: A peptide and B peptide were produced from the same host cells - **Incorrect**.
- Option B: A peptide and B peptide were produced from different host cells - **Correct**.
- Option C: A peptide and C peptide were produced from the same host cells - **Incorrect** (C peptide is not part of synthetic insulin).
- Option D: A, B, and C peptides were produced from different host cells - **Incorrect** (C peptide is not used in synthetic insulin).

8. **Conclusion:**
- The correct answer is that A peptide and B peptide were produced from different host cells.

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