Which one of the following restriction enzyme can cut `"Tet"^R` site of pBR322 ?

Options

A. Pvu I, Pst I
B. BamH I, Sal I
C. Pvu II
D. EcoR I

Answer

To solve the question regarding which restriction enzyme can cut the "Tet"^R site of pBR322, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**:
- pBR322 is a plasmid vector that contains genes for antibiotic resistance, specifically for ampicillin and tetracycline. The "Tet"^R site refers to the tetracycline resistance gene.

2. **Identify the Restriction Enzymes**:
- The options given are: PBU1, PST1, BAMH1, SAL1, PBU2, and E-COR1. We need to determine which of these enzymes can cut within the tetracycline resistance gene.

3. **Analyze the Restriction Sites**:
- According to the information provided, the restriction enzymes BAMH1 and SAL1 are located within the tetracycline resistance gene of pBR322. This means they can cut the "Tet"^R site.

4. **Eliminate Incorrect Options**:
- PBU1 and PST1 are associated with the ampicillin resistance gene, so they are not relevant to the "Tet"^R site.
- E-COR1 is also not associated with the tetracycline resistance gene, making it an incorrect option.
- PBU2 is not mentioned in relation to the tetracycline resistance site.

5. **Conclude the Correct Answer**:
- The restriction enzymes that can cut the "Tet"^R site of pBR322 are BAMH1 and SAL1.

### Final Answer:
The restriction enzymes that can cut the "Tet"^R site of pBR322 are **BAMH1 and SAL1**.