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If the gene of interest is introduced in...

If the gene of interest is introduced in a tetracycline - resistant gene region of pBR322, then:
(a) The host will become resistant for tetracycline
(b) The host will start producing tetracycline
(c) The host will start producing ampicillin.
(d) The host will loose resistance for antibiotic tetracycline.

A

The host will become resistant for tetracycline

B

The host will start producing tetracycline

C

The host will start producing ampicillin.

D

The host will loose resistance for antibiotic tetracycline.

Text Solution

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The correct Answer is:
To solve the question regarding the introduction of a gene of interest into the tetracycline-resistant gene region of pBR322, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding pBR322**: - pBR322 is a plasmid that contains genes for resistance to two antibiotics: tetracycline and ampicillin. It is commonly used in molecular cloning. 2. **Gene Insertion**: - When a gene of interest is inserted into a specific region of a plasmid, it can disrupt the function of that region. If the gene is inserted into the tetracycline resistance gene, it will interfere with the gene's ability to confer resistance. 3. **Effect on Tetracycline Resistance**: - The tetracycline resistance gene must remain intact to provide resistance to the antibiotic. Inserting a new gene into this region will disrupt its function, leading to a loss of resistance to tetracycline. 4. **Evaluating the Options**: - **(a)** The host will become resistant to tetracycline: **Incorrect**. The insertion disrupts the resistance gene. - **(b)** The host will start producing tetracycline: **Incorrect**. The host does not produce antibiotics; it can only be resistant to them. - **(c)** The host will start producing ampicillin: **Incorrect**. The host does not produce antibiotics; it can only be resistant to them. - **(d)** The host will lose resistance to antibiotic tetracycline: **Correct**. The insertion of the gene into the tetracycline resistance gene disrupts its function. 5. **Conclusion**: - The correct answer is (d) The host will lose resistance for antibiotic tetracycline.
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