In gel electrophoresis, differential mobility of DNA depends upon

Options

A. helical nature of DNA
B. double - stranded nature of DNA
C. charge and size of DNA
D. hydrogen honding between bases

Answer

**Step-by-Step Solution:**

1. **Understanding Gel Electrophoresis:**
Gel electrophoresis is a technique used to separate DNA fragments based on their size and charge. It involves placing DNA samples in a gel matrix and applying an electric current.

2. **Charge of DNA:**
DNA molecules are negatively charged due to their phosphate backbone. When an electric current is applied, the negatively charged DNA moves towards the positive electrode.

3. **Size of DNA:**
The size of the DNA fragments plays a crucial role in their movement through the gel. Smaller DNA fragments can move through the gel matrix more easily and quickly than larger fragments.

4. **Differential Mobility:**
The differential mobility of DNA in gel electrophoresis is primarily influenced by the charge and size of the DNA fragments. While other properties such as helical nature, double-stranded nature, and hydrogen bonding are characteristics of DNA, they do not affect how DNA moves through the gel.

5. **Conclusion:**
Therefore, the correct answer to the question is that the differential mobility of DNA in gel electrophoresis depends upon the charge and size of DNA.

**Final Answer:**
The differential mobility of DNA in gel electrophoresis depends upon the charge and size of DNA.

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