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Find the correct Statement ....

Find the correct Statement .

A

Ori means origin transcription

B

Some bacterial cell may have copy number of plasmids vary from 15-100

C

Vector should have many recognition sites for commonly used restriction enzyme so that alien DNA can attach to any one of the sites easily .

D

`tet^R` gene in pBR322 can be cleaved by pvu I and Pst I.

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question "Find the correct Statement," we will analyze each statement provided in the question and determine its validity step by step. ### Step 1: Analyze the First Statement **Statement:** ORI means origin transcription. **Analysis:** ORI stands for "Origin of Replication," not "Origin of Transcription." The origin of replication is crucial for a vector to replicate within a host cell. Therefore, this statement is **incorrect**. ### Step 2: Analyze the Second Statement **Statement:** Some bacterial cells may have a copy number of plasmid that varies from 15 to 100. **Analysis:** This statement is **correct**. The copy number of plasmids can indeed vary widely among different bacterial strains and plasmids, typically ranging from around 15 to 100 copies per cell, depending on various factors. ### Step 3: Analyze the Third Statement **Statement:** Vector should have many recognition sites for commonly used restriction enzymes so that alien DNA can attach to any one of the sites easily. **Analysis:** This statement is somewhat misleading. While it is true that vectors should have multiple recognition sites for different restriction enzymes, the phrasing suggests that a single restriction enzyme should have many recognition sites, which is not accurate. Therefore, this statement is **incorrect**. ### Step 4: Analyze the Fourth Statement **Statement:** Tetracycline resistance gene in PBR322 can be cleaved by PvuI and PSTI restriction enzymes. **Analysis:** This statement is **incorrect**. The tetracycline resistance gene in PBR322 is not cleaved by PvuI and PSTI; these enzymes are associated with the ampicillin resistance gene instead. ### Conclusion After analyzing all the statements, the only correct statement is: **Correct Statement:** Some bacterial cells may have a copy number of plasmid that varies from 15 to 100.
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The correct statement is

The correct statement is