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A virus with ssDNA is discovered. Its D...

A virus with ssDNA is discovered. Its DNA is amplified by running 19 cycles of PCR. How many dsDNA strand will be formed at the end ?
(a) 10
(b) 512
(c) 1024
(d) 102

A

10

B

512

C

1024

D

102

Text Solution

AI Generated Solution

The correct Answer is:
To solve the problem, we need to determine how many double-stranded DNA (dsDNA) strands will be formed after running 19 cycles of PCR on a single-stranded DNA (ssDNA) virus. ### Step-by-Step Solution: 1. **Understanding PCR**: - PCR (Polymerase Chain Reaction) is a method used to amplify DNA. Each cycle of PCR typically doubles the amount of DNA present. 2. **Initial Condition**: - We start with a single-stranded DNA (ssDNA). In the first cycle of PCR, this ssDNA will be converted into a double-stranded DNA (dsDNA). 3. **Cycles of PCR**: - In the first cycle, the ssDNA is converted into 1 dsDNA molecule. - From the second cycle onward, each dsDNA will serve as a template for further amplification. 4. **Calculating the Number of dsDNA after 19 Cycles**: - The formula for calculating the number of dsDNA strands formed in PCR is generally \(2^n\), where \(n\) is the number of cycles. - However, since the first cycle is used to convert ssDNA to dsDNA, we effectively have \(19 - 1 = 18\) cycles left for amplification. - Thus, we need to calculate \(2^{18}\) to find the total number of dsDNA strands formed after the 19 cycles. 5. **Calculating \(2^{18}\)**: - \(2^{18} = 262144\) dsDNA strands. 6. **Final Count**: - Since the first cycle was used to create the first dsDNA, we can say that after 19 cycles, we will have \(2^{18} = 262144\) dsDNA strands. ### Conclusion: The total number of double-stranded DNA strands formed at the end of 19 cycles of PCR is **262144**. ### Answer: None of the provided options (10, 512, 1024, 102) are correct based on the calculation. The correct answer is **262144**.
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